桫椤配子体发育与孢子体再生,Journal of Plant Growth Regulation
桫椤配子体发育与孢子体再生
Journal of Plant Growth Regulation
(
IF
3.9
)
Pub Date : 2023-05-19
, DOI:
10.1007/s00344-023-11026-8
Yuping Xiong
,
Yujie Zeng
,
Junyu Liu
,
Xiaohong Chen
,
Yuan Li
,
Xinhua Zhang
,
Zhan Bian
,
Jaime A. Teixeira da Silva
,
Songjun Zeng
,
Kunlin Wu
,
Guohua Ma
Alsophila spinulosa (Wall. ex Hook) Tryon 是地球上为数不多的树蕨之一。作为活的中生代蕨类植物化石，对于研究古气候和古环境变化具有重要意义。A. spinulosa也具有相当的观赏和药用价值。本研究评估了A. spinulosa中的体外孢子萌发、配子体发育和孢子体再生并建立了配子体增殖、愈伤组织诱导和孢子体再生的有效方案。还研究了几种植物生长调节剂 (PGR) 及其浓度对配子体发育和孢子体形成的影响。在无 PGR 的 Murashige 和 Skoog (MS) 培养基上没有观察到形态学变化，尽管 MS 培养基中 PGRs 的存在在细丝和原叶体的发育阶段引起了一些变化。当每个原叶体表面形成细丝时，原叶体就可以增殖。在含有 1.0 mg L -1 6-苄基腺嘌呤的 MS 培养基上，30 天内原叶体的最高增殖系数 (PPC) 为 6.6 。孢子体很容易在无 PGR 的 MS 培养基或含 0.2–1.0 mg L −1的 MS 培养基中生根吲哚-3-丁酸和/或 α-萘乙酸，最低 93% 的生根率。将幼根孢子体转移到蛭石和泥炭基质后，一个月内的存活率为 92.7%（两个月后为 88.7%）。在含有 0.2 mg L −1 2,4-二氯苯氧乙酸和 0.5 mg L −1 噻二唑隆的 MS 培养基上，基于孢子体成功诱导愈伤组织并形成不定芽。每个愈伤组织簇平均可分化出13.6个孢子体。
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Gametophyte Development and Sporophyte Regeneration of Alsophila spinulosa
Alsophila spinulosa (Wall. ex Hook) Tryon is one of the few remaining tree ferns on Earth. As a living Mesozoic fern fossil, it has great significance for studies on paleo-climate and paleo-environmental changes. A. spinulosa also has considerable ornamental and medicinal value. This study assessed in vitro spore germination, gametophyte development and sporophyte regeneration in A. spinulosa and established efficient protocols for gametophyte proliferation, callus induction and regeneration from sporophytes. The effects of several plant growth regulators (PGRs) and their concentrations on gametophyte development and sporophyte formation were also investigated. No morphological variations were observed on PGR-free Murashige and Skoog (MS) medium although the presence of PGRs in MS medium induced some variations during the development stages of filaments and prothallia. When filaments formed on the surface of each prothallium, prothallia could be proliferated. Highest prothallium proliferation coefficient (PPC) was 6.6 within 30 days on MS medium with 1.0 mg L−1 6-benzyladenine. Sporophytes readily formed roots on PGR-free MS medium or in MS medium with 0.2–1.0 mg L−1 indole-3-butyric acid and/or α-naphthaleneacetic acid, with a minimal 93% rooting. After young rooted sporophytes were transferred to a vermiculite and peat substrate, survival percentage was 92.7% within one month (and 88.7% after two months). Callus was successfully induced and adventitious shoots formation from sporophyte base on MS medium with 0.2 mg L−1 2,4-dichlorophenoxyacetic acid and 0.5 mg L−1 thidiazuron. Each callus cluster could differentiate into mean 13.6 sporophytes.
更新日期：2023-05-19